Pathology of explanted polytetrafluoroethylene vascular grafts
Received 14 December 2009; received in revised form 26 May 2010; accepted 3 June 2010. published online 12 July 2010. Corrected Proof
Abstract
Introduction
Graft occlusion is a well-documented etiology for arteriovenous fistulae failure. However, there is little morphologic information elucidating why synthetic vascular grafts fail. The purpose of this study was to examine the tissue responses occurring within and adjacent to explanted polytetrafluoroethylene grafts that were utilized during cardiovascular procedures and subsequently removed.
Methods
Forty explanted polytetrafluoroethylene grafts (including 32 failed vascular grafts) originating from 18 females and 22 males who ranged in age from 6 to 82 years (mean age, 36 years) were evaluated. Duration of engraftment varied from 1 to 255 months (mean engraftment period, 64 months).
Results
In addition to neointimal hyperplasia, foreign body reaction, and thrombosis, an unexpected finding was calcification involving the graft material, as well as luminal thrombus and adjacent soft tissues. Twenty-seven of forty cases (68%) showed evidence of calcification, either within or adjacent to polytetrafluoroethylene grafts. Histologic examination revealed variable degrees and patterns of calcification within and adjacent to explanted polytetrafluoroethylene membranes and conduits (arterial, arteriovenous, or cardiac grafts). A significantly longer duration of engraftment (P=.015) was identified in calcified versus noncalcified polytetrafluoroethylene materials. Patient age, serum calcium, creatinine level, and blood urea nitrogen level showed no statistically significant differences between patients with calcified grafts and patients without calcified grafts.
Conclusions
Interstitial calcification is frequently found within explanted polytetrafluoroethylene grafts and is associated with graft disruption. These findings suggest that calcification of polytetrafluoroethylene biomaterials may play a role in eventual graft failure. A better understanding of the process of polytetrafluoroethylene graft calcification may lead to novel therapies that aid in the prevention of polytetrafluoroethylene vascular graft failure.
aDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles, CA 90095, USA
bDepartment of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, CA 90095, USA
cC-Link Micro Imaging, Inc., Los Angeles, CA 90035, USA
Corresponding author. 10833 Le Conte Avenue, 18-126 CHS, Los Angeles, CA 90095, USA. Tel.: +1 310 825 0544; fax: +1 310 206 8290.
Disclosure of interest: the authors have no conflicts of interest to disclose.
This work was supported through generous funds from the Oppenheimer Program Clinical Translational Seed Grant (R.I.M.), and the Piansky Family Trust (M.C.F.).
ABSTRACT