Cardiovascular Pathology
Volume 19, Issue 4 , Pages e91-e98 , July 2010

Reduced cyclic stretch, endothelial dysfunction, and oxidative stress: an ex vivo model

  • Tyler Thacher

      Affiliations

    • LHTC, Institute of Bioengineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland
    • Corresponding Author InformationCorresponding author. Laboratiore d'Hémodynamique et de Technologie Cardiovasculaire, École Polytechnique Fédéral de Lausanne, Bâtiment AI 1241, Station 15, 1015 Lausanne, CH, Switzerland. Tel.: +41 21 693 9654; fax: +41 21 693 9635.
    • ,
  • Veronica Gambillara

      Affiliations

    • LHTC, Institute of Bioengineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland
    • ,
  • Rafaela F. da Silva

      Affiliations

    • LHTC, Institute of Bioengineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland
    • Neurovascular Lab, Department of Neurosurgery, Geneva University Medical Center and Faculty of Medicine, University of Geneva, Geneva, Switzerland
    • ,
  • Paolo Silacci

      Affiliations

    • LHTC, Institute of Bioengineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland
    • ,
  • Nikos Stergiopulos

      Affiliations

    • LHTC, Institute of Bioengineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland

Received 9 January 2009 ,Revised 23 June 2009 ,Accepted 30 June 2009.

References 

  1. Zieman SJ, Melenovsky V, Kass DA. Mechanisms, pathophysiology, and therapy of arterial stiffness. Arterioscler Thromb Vasc Biol. 2005;25:932–943
  2. Lehoux S, Tedgui A. Signal transduction of mechanical stresses in the vascular wall. Hypertension. 1998;32:338–345
  3. Haider AW, Larson MG, Franklin SS, Levy D. Systolic blood pressure, diastolic blood pressure, and pulse pressure as predictors of risk for congestive heart failure in the Framingham Heart Study. Ann Intern Med. 2003;138:10–16
  4. Valko M, Leibfritz D, Moncol J, Cronin MT, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39:44–84
  5. Brooks AR, Lelkes PI, Rubanyi GM. Gene expression profiling of vascular endothelial cells exposed to fluid mechanical forces: relevance for focal susceptibility to atherosclerosis. Endothelium. 2004;11:45–57
  6. Manea A, Manea SA, Gafencu AV, Raicu M. Regulation of NADPH oxidase subunit p22(phox) by NF-kB in human aortic smooth muscle cells. Arch Physiol Biochem. 2007;113:163–172
  7. Babior BM. NADPH oxidase: an update. Blood. 1999;93:1464–1476
  8. Michell BJ, Harris MB, Chen ZP, Ju H, et al. Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem. 2002;277:42344–42351
  9. Bae SW, Kim HS, Cha YN, Park YS, et al. Rapid increase in endothelial nitric oxide production by bradykinin is mediated by protein kinase A signaling pathway. Biochem Biophys Res Commun. 2003;306:981–987
  10. Gambillara V, Montorzi G, Haziza-Pigeon C, Stergiopulos N, Silacci P. Arterial wall response to ex vivo exposure to oscillatory shear stress. J Vasc Res. 2005;42:535–544
  11. Montorzi G, Silacci P, Zulliger M, Stergiopulos N. Functional, mechanical and geometrical adaptation of the arterial wall of a non-axisymmetric artery in vitro. J Hypertens. 2004;22:339–347
  12. Gambillara V, Chambaz C, Montorzi G, Roy S, et al. Plaque-prone hemodynamics impair endothelial function in pig carotid arteries. Am J Physiol Heart Circ Physiol. 2006;290:H2320–H2328
  13. Kwak BR, Silacci P, Stergiopulos N, Hayoz D, Meda P. Shear stress and cyclic circumferential stretch, but not pressure, alter connexin43 expression in endothelial cells. Cell Commun Adhes. 2005;12:261–270
  14. Brandes RP, Kreuzer J. Vascular NADPH oxidases: molecular mechanisms of activation. Cardiovasc Res. 2005;65:16–27
  15. Kakisis JD, Liapis CD, Sumpio BE. Effects of cyclic strain on vascular cells. Endothelium. 2004;11:17–28
  16. Noris M, Morigi M, Donadelli R, Aiello S, et al. Nitric oxide synthesis by cultured endothelial cells is modulated by flow conditions. Circ Res. 1995;76:536–543
  17. Qiu Y, Tarbell JM. Interaction between wall shear stress and circumferential strain affects endothelial cell biochemical production. J Vasc Res. 2000;37:147–157
  18. Peng X, Haldar S, Deshpande S, Irani K, Kass DA. Wall stiffness suppresses Akt/eNOS and cytoprotection in pulse-perfused endothelium. Hypertension. 2003;41:378–381
  19. Li Y, Zheng J, Bird IM, Magness RR. Effects of pulsatile shear stress on signaling mechanisms controlling nitric oxide production, endothelial nitric oxide synthase phosphorylation, and expression in ovine fetoplacental artery endothelial cells. Endothelium. 2005;12:21–39
  20. Li Y, Zheng J, Bird IM, Magness RR. Mechanisms of shear stress-induced endothelial nitric-oxide synthase phosphorylation and expression in ovine fetoplacental artery endothelial cells. Biol Reprod. 2004;70:785–796
  21. Ziegler T, Silacci P, Harrison VJ, Hayoz D. Nitric oxide synthase expression in endothelial cells exposed to mechanical forces. Hypertension. 1998;32:351–355
  22. Li M, Chiou KR, Bugayenko A, Irani K, Kass DA. Reduced wall compliance suppresses Akt-dependent apoptosis protection stimulated by pulse perfusion. Circ Res. 2005;97:587–595
  23. Yang Z, Kaye DM. Endothelial dysfunction and impaired l-arginine transport in hypertension and genetically predisposed normotensive subjects. Trends Cardiovasc Med. 2006;16:118–124
  24. Yung LM, Leung FP, Yao X, Chen ZY, Huang Y. Reactive oxygen species in vascular wall. Cardiovasc Hematol Disord Drug Targets. 2006;6:1–19
  25. Skyschally A, Schulz R, Gres P, Korth HG, Heusch G. Attenuation of ischemic preconditioning in pigs by scavenging of free oxyradicals with ascorbic acid. Am J Physiol Heart Circ Physiol. 2003;284:H698–H703
  26. Paravicini TM, Touyz RM. Redox signaling in hypertension. Cardiovasc Res. 2006;71:247–258
  27. Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002;82:47–95
  28. Paravicini TM, Touyz RM. NADPH oxidases, reactive oxygen species, and hypertension: clinical implications and therapeutic possibilities. Diabetes Care. 2008;31(Suppl 2):S170–S180
  29. Gambillara V, Thacher T, Silacci P, Stergiopulos N. Effects of reduced cyclic stretch on vascular smooth muscle cell function of pig carotids perfused ex vivo. Am J Hypertens. 2008;
  30. Griendling KK, Sorescu D, Ushio-Fukai M. NAD(P)H oxidase: role in cardiovascular biology and disease. Circ Res. 2000;86:494–501

 This work was supported by the Swiss National Science Foundation: grant 310000118274.

PII: S1054-8807(09)00074-X

doi: 10.1016/j.carpath.2009.06.007

Cardiovascular Pathology
Volume 19, Issue 4 , Pages e91-e98 , July 2010

Article Tools

* Image Usage & Resolution